This
invention is a new protein transduction method for efficient delivery of
exogenous proteins into mammalian cells. In contrast to known protein
transduction methods, this new approach has the capability of targeting
transduced proteins to different cellular compartments, as well as protecting
the proteins from degradation by cellular proteases. The reagent for delivery of
the proteins is composed of cationic agents, lipids and enhancer in a carrier.
The Approach uses bacteria to produce recombinant proteins that are then
modified with the reagent and delivered into mammalian cells. The mammalian cell
machinery folds and post-translationally modifies the transduced proteins to
produce native functional proteins that can be easily purified from the cells.
The final high yield of properly folded and modified protein can be 35-60% of
added bacterial expressed proteins. This discovery provides a foundation for
future applications of protein transduction technology, atomic resolution cell
biology and protein drug therapy to treat human disease to name a few
applications.
Commercial
Applications
·
Extend
Structural/ Folding studies of proteins in living
cells
·
Prepare
proteins with correct-transcriptional
modifications
·
Propare
properly folded proteins with a large quantity for every single
protein
Competitive
Advantages
·
Solves
the major folding problem of bacterial expression, by using both high-level
bacterial expression and powerful folding machinery of the machinery of the
mammalian cells
·
Ability
to produce milligram quantity properly folded proteins, which is impossible to
obtain using only bacterial expression
Patent
Status
A patent
application is on file.
Tech
ID
07-830