Detailed Description
WSU researchers have developed a fluorescence-based
assay for the dealkylation activity of the non-heme iron (II)
a-ketoglutarate-dependent dioxygenase family of enzymes. This assay uses
formaldehyde dehydrogenase to convert formaldehyde to formic acid and monitors
the creation of an NADH analog using fluorescence. The technology is a great
improvement over the existing assays for DNA demethylation in that is continuous
rapid and does not require radioactively labeled material. Additionally this
assay may also be utilized to study the demethylation of histones. This
invention has been reported in T. W. Roy and A. S. Bhagwat ?Kinetic studies of
E. coli AlkB using a new fluorescence-based assay for DNA demethylation? Nucleic
Acids Research 2007 Vol. 35 No.21.
File Number: 08-904
