A novel ATP analog featuring a polyamine linker enables efficient kinase-catalyzed biotinylation within live cells by enhancing cell permeability.
Technology Summary
ATP (adenosine 5’-triphosphate) analogs such as ATP-biotin are widely used to monitor biochemical events in vitro. WSU researchers developed a specially designed ATP analog that replaces the conventional PEG linker with a positively charged polyamine linker, significantly improving cell permeability. This advancement allows the ATP analog to enter live cells and participate in kinase-catalyzed biotinylation, enabling detailed study of protein kinases and their substrates in physiologically relevant conditions. Experimental validation demonstrates successful intracellular protein labeling, overcoming the limitations of traditional ATP-biotin which is restricted to in vitro use. The invention supports enhanced research into cell signaling pathways critical to diseases such as cancer and diabetes.
Key Advantages
- Enhanced cell permeability.
- Improves labeling efficiency and physiological relevance of protein interaction studies.
- Enables real-time study of kinase activity and substrate interactions inside living cells.
- Detailed chemical variations and synthesis methods for application flexibility.
Market Opportunities
- Facilitates advanced research into signaling pathways and disease mechanisms.
- Biochemical and molecular biology research focused on kinase function.
- Cell signaling pathway analysis in kinase-related diseases including cancer, diabetes, and others.
- Development of diagnostic tools based on kinase activity and protein labeling.
Stage of Development
Pre-Clinical.
Patent Status
Issued US patent 10,429,397
References & Publications
Fouda 2015, Angew Chem Int Ed. 54(33), 9618-21
https://doi.org/10.1002/anie.201503041
